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A antimicrobial resistance animated video developed by FDA.

Source: Kerrie Armstrong, SBS
It may sound too sweet to be true, but research is underway to see whether honey could replace some antibiotic treatments.

If you had told Professor Liz Harry she would one day be researching honey as an antibiotic alternative she would have laughed.

But in the battle against increasing antibiotic resistance in Australia and around the world, honey could be the new secret weapon.

The medicinal use of honey has gone from a quirky alternative medicine to a serious research project – and Professor Harry is at its forefront.

She is the acting director of the ithree institute (infection, immunology and innovation) at the University of Technology, Sydney and is working as part of the Australian Honey Project, which is connected to the Rural Industry Research Development Corporation.
She is looking into the practical use of honey as an alternative to topical antibiotics.

“It doesn’t matter what antibiotics are used, bugs will always become resistant to it,” Professor Harry told SBS News.

“Honey has multiple antibiotic components in it.

“It doesn’t allow bacteria to rescue themselves or have a chance of surviving.”

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Professor Harry said the use of honey as a medicine was not new.

“I didn’t realise honey was used since the dawn of time and was used up until the discovery of antibiotics,” she said.

Evidence has been found that even ancient cultures like the ancient Egyptians were convinced on honey’s healing powers.

She said her research aims to prove whether honey is effective at targeting a range of bacteria and whether is able to remain effective over long term use.

It is then used as a topical treatment – that is, treating wounds and infections on the skin – either as a dressing or in the form of a cream or a gel.

She said there had been considerable success in the use of honey this way, as well as using topical honey treatments in conjunction with oral antibiotics.

The honey is also able to remove dead cells from a wound, which is known as debriding the wound.
Professor Harry said she hoped patients and medical professionals would one day consider honey to be an alternative wound or post-operative treatment to the antibiotic creams and gels that were currently used by general practitioners and in hospitals.

“It would help to reduce the resistance to antibiotics,” she said.

“One of reasons was have this problem is we use a lot of antibiotics.”

Certain honey gels and creams have been available for a number of years, Professor Harry said but more research is still needed into why honey works the way it does.

The honey project is expected to be completed in October, 2019.

Source: Laboratory News
A team of Bristolian scientists have started a large-scale study to investigate how antimicrobial resistant organisms can be transferred from farm animals to humans.
A main part of the project — ‘antimicrobial resistance (AMR) in the real world’ — will investigate if bacteria from cattle can cause drug resistant infections in humans. Half a million people, from Bristol and the surrounding areas, will be studied to see how many urine infections are caused by AMR bacteria found in dairy cows.
Dr Matthew Avison, from Bristol University’s School of Cellular & Molecular Medicine, said: “There is little doubt that over-using antibiotics in farm animals and pets increases the number of AMR bacteria in those animals, just as it does in humans. There is also strong evidence that AMR bacteria present in farm animals can spread to humans having close physical contact with them, for example, farm workers.
“However, there is considerable debate about the extent that AMR bacteria can spread more widely – for example when people eat food contaminated with bacteria from animals or interact with environments contaminated with animal wastes. Our research project will add much needed data to the debate.”
The researchers will also look at ways to reduce AMR bacteria levels in animals as part of the project. The scientists and government bodies will work with farmers, vets and retailers to encourage responsible use of antibiotics. The study will also investigate AMR bacteria in puppies by testing their faeces before they start going outside and after several months of walking along public footpaths. The last part of this multi-faceted study will also look at determining the effect of antibiotic use in humans on AMR bacteria levels and how a reduction in antibiotics affects this.
Professor Alistair, from the University’s School of Social and Community Medicine said: “We will look to see if these reductions in antibiotic prescribing are translating into reduced rates of antibiotic resistant urine infections. If we find that a reduction in antibiotic prescribing has led to a reduction in the number of antibiotic resistant urine infections, it will be positive news for patients, GPs and nurses in primary care.”
The research is funded by a £1.75m grant from the Natural Environment Research Council, the Biotechnology and Biological Sciences Research Council and the Medical Research Council.

Source:
Trinity Science Daily

Scientists have isolated peptides (strings of amino acids) with antibiotic effects on bacteria that spoil food and cause food poisoning, after turning to the plant kingdom for help in boosting our arsenal in the ongoing war against antibiotic resistance.

The scientists found two small peptides from widely cultivated crop species (one from broad beans and one from cowpea) that were especially effective.

Further work then confirmed that when these peptides were used together, and with a human peptide that is also an antimicrobial, their protective effects were beefed-up in a one-two antimicrobial punch.

Associate Professor and Head of Microbiology at Trinity College Dublin, Ursula Bond, led the team that has just published its research in the journal Applied and Environmental Microbiology.

She said: “There are two major advantages to these small peptides in that no resistance mechanisms have emerged yet, and in that they can be inexpensively synthesised in the lab. Initially, our aim was to identify peptides that provide protection against food-spoiling bacteria, but these peptides may also be useful as antibiotics against bacteria that cause serious human diseases.”

The research team behind the discovery had previously isolated a human peptide that is a potent antimicrobial agent against many of the bacteria that spoil beer during industrial fermentation. Instead of screening for other human peptides with similar desired effects, the scientists scanned plant peptides databases and focused on the peptides whose structural blueprints were similar to the human one with the desired characteristics.

Many of the most effective antibiotics are derived from proteins produced by plants, but there is a growing need to discover new therapeutic candidates as resistance is increasing in bacterial species that have major health and economic implications for society.

Professor Bond added: “We reasoned that natural peptides found in many plants and plant seeds might be useful new antibiotics, because plants have evolved these systems to protect themselves against the billions of bacteria and fungi they interact with in the soil every day.”

Story Source:

The above post is reprinted from materials provided by Trinity College Dublin. Note: Materials may be edited for content and length.

Source: drugtargtreview
At ASM Microbe 2016, Theravance Biopharma announced new positive data from several studies of Vibativ (telavancin) showing potent in vitro activity against isolates from a range of difficult-to-treat infections, including methicillin-resistant Staphylococcus aureus (MRSA).

Vibativ

We caught up with Frank Pasqualone, Senior Vice President and Global Head, Acute Care Business at Theravance Biopharma, to find out more about the therapy and the results of the studies. Mr. Pasqualone began by explaining more about the mode of action of the treatment: “Vibativ has a dual mechanism of action that works by both inhibiting bacterial cell wall synthesis and disrupting bacterial cell membrane function. This dual mechanism of action acts at two separate targets on the bacterium, differentiating Vibativ from several MRSA antibiotics, including vancomycin, the most commonly prescribed treatment for MRSA and related bacterial infections.”

Greater in vitro potency

The findings presented at ASM Microbe demonstrated that Vibativ possesses greater in vitro potency against MRSA and other difficult-to-treat clinical pathogens compared to widely prescribed antibiotics such as vancomycin, daptomycin and linezolid. We asked Mr. Pasqualone how the therapy differs from other antibiotics like vancomycin: “One of the most important points of comparison is the data presented at ASM Microbe which demonstrated greater in vitro potency for Vibativ against a range of difficult-to-treat Gram-positive pathogens as compared to other well-known antibiotics such as vancomycin, daptomycin and linezolid. These findings further supplement the extensive and well-documented evidence of greater in vitro potency for Vibativ against these types of infections as compared to alternative antibiotic treatments.

“Additionally, Vibativ is differentiated from vancomycin in a number of ways, including its dual mechanism of action, demonstrated penetration into infection sites, convenient, once-daily dosing with no need for therapeutic drug-level monitoring, ability to reduce MRSA concentrations within eight hours and the fact that no resistance to treatment was seen during its Phase II and III clinical programmes (reports of resistance during post-approval clinical use are extremely rare).”

Data at ASM Microbe 2016

The data presented at ASM Microbe showed that Vibativ possessed the greatest in vitro activity of all antibiotics evaluated against a broad, global collection of contemporary S. aureus clinical isolates causing bacteremia, including endocarditis. Overall, the minimum inhibitory concentrations (MICs) for Vibativ were eight times lower than for daptomycin and 16- to 32-times lower than for vancomycin and linezolid against the S. aureus isolates that were evaluated, including MRSA, MSSA and multi-drug resistant subsets.

Data from a second study demonstrated potent in vitro activity for Vibativ against multiple daptomycin-resistant MRSA strains causing infective endocarditis in a rigorous animal model. Vibativ significantly reduced the levels of MRSA found in all three target tissues (heart, kidney and spleen) that were evaluated as compared to the untreated control and daptomycin-treated groups. Additionally, Vibativ produced a high percentage of target tissues that were classified as culture-negative for MRSA, while daptomycin did not sterilize any of the target tissues. Finally, there was no mortality observed in animals treated with Vibativ, as opposed to a 29 percent mortality rate for those animals in the standard daptomycin treatment group.

Findings from a third study demonstrated potent in vitro activity for Vibativ against a broad, global collection of contemporary Gram-positive pathogens, including S. aureus clinical isolates such as MRSA, causing bone and joint infections. This potency was demonstrated against all S. aureus isolates evaluated, regardless of phenotype. Additionally, all clinical isolates that were shown to be daptomycin-resistant or teicoplanin-resistant remained susceptible to Vibativ.

Potency regardless of isolate phenotype and resistance profile

Mr. Pasqualone commented on the ASM Microbe data: “These findings demonstrated greater in vitro activity for Vibativ against a range of difficult-to-treat Gram-positive pathogens.

“Importantly, the in vitro activity of Vibativ was demonstrated against isolates from difficult-to-treat infection types with significant unmet medical need including Staphylococcus aureus bacteremia, infective endocarditis caused by daptomycin-resistant MRSA, and bone and joint infections. In some cases, these pathogens against which Vibativ demonstrated in vitro potency were non-susceptible or resistant to treatment with other evaluated antibiotics.

“As we continue to evaluate Vibativ against a range of these infection-causing clinical isolates, we are impressed to consistently see that the drug has potent in vitro activity, regardless of the isolates’ phenotype and resistance profile. Importantly, in many cases this potency is demonstrated to be several fold greater than other antibiotics routinely used for the treatment of Gram-positive infections.”

Antimicrobial resistance

The threat of antibiotic resistance is great. Over the last thirty years the number of new antibiotic approvals has dropped and the number of effective antibiotics has decreased. Jim O’ Neill’s Review on Antimicrobial Resistance (AMR) highlights that drug resistance has serious implications and will be felt the world over: routine surgeries and minor infections will become life-threatening once again, and hospital stays/expenses will increase significantly. The review notes that infections are on the rise and by 2050 the number of deaths from drug resistant infections is predicted to surpass the number of deaths from cancer.

We asked Mr. Pasqualone more about the threat of antimicrobial resistance and how Vibativ can help combat it: “With the crisis of antibiotic resistance continuing to grow more acute, there is an urgent need for differentiated antibiotic products that are able to address difficult-to-treat bacteria that have limited or no susceptibility to currently available antibiotics. We believe that Vibativ demonstrates a number of critical attributes that may offer key advantages in the fight against antibiotic resistance. These include demonstrated in vitro potency, dual mechanism of action, clinical evidence of efficacy in a range of difficult-to-treat infections in HABP/VABP and cSSSI, proven ability to penetrate important lung and tissue sites, and dosing that ensures effective levels of the antibiotic are maintained.”